Scientific Publications
GMP Manufacturing
2020-09-03 - Vaccine
First-in-human administration of a live-attenuated RSV vaccine lacking the G-protein assessing safety, tolerability, shedding and immunogenicity: a randomized controlled trial
Pauline Verdijk , Johan L van der Plas, Emilie M J van Brummelen, Rienk E Jeeninga, Cornelis A M de Haan, Meta Roestenberg, Jacobus Burggraaf , Ingrid M C Kamerling
pubmed.ncbi.nlm.nih.gov
2014-09-22 - Vaccine
Reactogenicity and immunogenicity of inactivated poliovirus vaccine produced from Sabin strains: a phase I Trial in healthy adults in Cuba
Sonia Resik , Alina Tejeda, Magilé Fonseca, Nilda Alemañi, Manuel Diaz, Yenisleidys Martinez, Gloria Garcia, Hiromasa Okayasu, Anthony Burton , Wilfried A M Bakker, Pauline Verdijk, Roland W Sutter
2014-09-03 - Vaccine
Safety and immunogenicity of a primary series of Sabin-IPV with and without aluminum hydroxide in infants
Pauline Verdijk, Nynke Y Rots, Monique G C T van Oijen, William C Weldon, M Steven Oberste, Hiromasa Okayasu, Roland W Sutter, Wilfried A M Bakker
Formulation and adjuvants
2022-06-10 - microLife
Lipid A heterogeneity and its role in the host interactions with pathogenic and commensal bacteria
Sukumar Saha, Elder Pupo, Afshin Zariri, Peter van der Ley
2022-04-01 - Journal of Pharmaceutical Sciences
Aluminum Hydroxide And Aluminum Phosphate Adjuvants Elicit A Different Innate Immune Response
Sietske Kooijman, Hilde Vrieling, Lisa Verhagean, Justin de Ridder, Alex de Haan, Elly van Riet , Albert J.R. Heck, Gideon F.A. Kersten, Jeroen L.A. Pennings, Bernard Metz, Hugo D. Meiring
2022-03-21 - Frontiers in Immunology
Activation of Canine, Mouse and Human TLR2 and TLR4 by Inactivated Leptospira Vaccine Strains
Andreja Novak, Elder Pupo, Esther Van’t Veld, Victor P M G Rutten, Femke Broere, Arjen Sloots
2022-03-17 - ACS American Chemical Society
The First-in-Human Synthetic Glycan-Based Conjugate Vaccine Candidate against Shigella
Robert M. F. van der Put, Carolien Smitsman, Alex de Haan, Martin Hamzink, Hans Timmermans, Joost Uittenbogaard, Janny Westdijk, Michiel Stork, Olga Ophorst, Françoise Thouron, Catherine Guerreiro, Philippe J. Sansonetti, Armelle Phalipon, and Laurence A.
2021-12-01 - Analytical Chemistry
Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides
Elder Pupo, Peter van der Ley, Hugo Meijring
2020-07-14 - Scientific Reports
Formaldehyde treatment of proteins enhances proteolytic degradation by the endo-lysosomal protease cathepsin S
Thomas J M Michiels, Hugo D Meiring, Wim Jiskoot, Gideon F A Kersten, Bernard Metz
A great number of laboratory animals are still being used all over the world for the quality control of vaccines. This also applies for tetanus and diphtheria vaccines, both consisting of bacterial toxins inactivated by formaldehyde. This inactivation largely determines the quality of these vaccines. Researchers from Intravacc and LACDR have been searching for a test that reduces the use of laboratory animals. For more information, see our press release
2020-04-15 - European Journal of Pharmaceutical Sciences
Hyaluronan molecular weight: Effects on dissolution time of dissolving microneedles in the skin and on immunogenicity of antigen
Mara Leone, Stefan Romeijn, Bram Slütter, Conor O’Mahony, Gideon Kersten, Joke A Bouwstra
2020-01-01 - Journal of Pharmaceutical Science
Activation of Human Monocytes by Colloidal Aluminum Salts
Hilde Vrieling, Sietske Kooijman, Justin W de Ridder, Dominique M E Thies-Weesie, Peter C Soema, Wim Jiskoot, Elly van Riet, Albert J R Heck, Albert P Philipse, Gideon F A Kersten, Hugo D Meiring, Jeroen L Pennings, Bernard Metz
2019-10-01 - Journal of Controlled Release
Skin delivery of trivalent Sabin inactivated poliovirus vaccine using dissolvable microneedle patches induces neutralizing antibodies
Agnese Donadei, Heleen Kraan, Olga Ophorst, Olivia Flynn, Conor O’Mahony, Peter C Soema, Anne C Moore
2019-09-01 - Colloids and Surfaces B: Biointerfaces
Stabilised aluminium phosphate nanoparticles used as vaccine adjuvant
Hilde Vrieling , Margarita Espitia Ballestas, Martin Hamzink, Geert-Jan Willems, Peter Soema, Wim Jiskoot, Gideon Kersten, Bernard Metz
Platforms Cell based
2023-11-28 - Plos
Automated cell counting for Trypan blue-stained cell cultures using machine learning
Louis Kuijpers, Edo van Veen, Leo A. van der Pol, Nynke H. Dekker,
2021-07-01 - Scientific Reports
Development of an animal component free production process for Sabin inactivated polio vaccine
Patricia Kaaijk, Maarten E. Emmelot, Hugo D. Meiring, Cécile A. C. M. van Els, Jelle de Wit
2021-07-01 - Scientific Reports
Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection
Patricia Kaaijk, Maarten E. Emmelot, Hugo D. Meiring, Cécile A. C. M. van Els, Jelle de Wit
2021-06-25 - Vaccines
Genetic Analysis Reveals Differences in CD8+ T Cell Epitope Regions That May Impact Cross-Reactivity of Vaccine-Induced T Cells against Wild-Type Mumps Viruses
Patricia Kaaijk, Maarten E. Emmelot, Jeroen Kerkhof, Cécile A.C.M. van Els, Hugo D. Meiring, Jelle de Wit, Rogier Bodewes
2020-09-03 - Vaccines
First-in-human administration of a live-attenuated RSV vaccine lacking the G-protein assessing safety, tolerability, shedding and immunogenicity: a randomized controlled trial
Pauline Verdijk , Johan L van der Plas, Emilie M J van Brummelen, Rienk E Jeeninga, Cornelis A M de Haan, Meta Roestenberg, Jacobus Burggraaf , Ingrid M C Kamerling
2019-11-08 - Vaccines
Accelerating bioprocess development by analysis of all available data
Yang Jiang, Joyce E van der Welle, Olaf Rubingh, Gerco van Eikenhorst, Wilfried A M Bakker, Yvonne E Thomassen
2019-10-01 - Journal of Controlled Release
Skin delivery of trivalent Sabin inactivated poliovirus vaccine using dissolvable microneedle patches induces neutralizing antibodies
Agnese Donadei, Heleen Kraan, Olga Ophorst, Olivia Flynn, Conor O’Mahony, Peter C Soema, Anne C Moore
2019-06-01 - Process Biochemistry
Kinetic model for adherent Vero cell growth and poliovirus production in batch bioreactors
Yang Jiang, Joyce E van der Welle, Olaf Rubingh, Gerco van Eikenhorst, Wilfried A M Bakker, Yvonne E Thomassen
2016-11-01 - Biologicals
Development of inactivated poliovirus vaccine from Sabin strains: A progress report
Hiromasa Okayasu , Carolyn Sein, Ahd Hamidi, Wilfried A M Bakker, Roland W Sutter
2022-10-12 - Viruses
Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines
Wouter Johannes Petrus van den Braak, Bella Monica, Diana Limpens, Dedeke Rockx-Brouwer, Matthijn de Boer, Dinja Oosterhoff
Platforms Conjugation
2023-01-19 - Vaccines
Carriers and Antigens: New Developments in Glycoconjugate Vaccines
Robert M.F. van der Put, Bernard Metz, Roland J. Pieters
2021-04-01 - The Lancet. Infectious Diseases
Safety and immunogenicity of a synthetic carbohydrate conjugate vaccine against Shigella flexneri 2a in healthy adult volunteers: a phase 1, dose-escalating, single-blind, randomised, placebo-controlled study
Dani Cohen , Jacob Atsmon, Cécile Artaud, Shiri Meron-Sudai, Marie-Lise Gougeon, Anya Bialik, Sophy Goren, Valeria Asato, Ortal Ariel-Cohen, Arava Reizis, Alexandra Dorman, Carla W G Hoitink, Janny Westdijk, Shai Ashkenazi, Philippe Sansonetti, Laurence A
The results of a clinical study with an experimental vaccine against Shigella, intestinal bacteria that cause severe diarrhea and dysentery. The paper was authored by a team of researchers and clinicians at Tel Aviv University, Tel Aviv Sourasky Medical Center, Institut Pasteur, Ariel University and Intravacc. The clinical study in healthy adults shows that the vaccine is well tolerated and immunogenic. Intravacc developed a production process for this conjugate vaccine, manufactured the GMP batch and did stability testing and batch release. For more information, see also our press release
2021-03-17 - ACS Central Science
The First-in-Human Synthetic Glycan-Based Conjugate Vaccine Candidate against Shigella
Robert M F van der Put, Carolien Smitsman, Alex de Haan, Martin Hamzink, Hans Timmermans, Joost Uittenbogaard, Janny Westdijk, Michiel Stork, Olga Ophorst, Françoise Thouron, Catherine Guerreiro, Philippe J Sansonetti Armelle Phalipon, Laurence A Mulard
2016-05-01 - Biotechnology Progress
Process development of a New Haemophilus influenzae type b conjugate vaccine and the use of mathematical modeling to identify process optimization possibilities
Ahd Hamidi, Hans Kreeftenberg, Leo V D Pol, Saroj Ghimire, Luuk A M V D Wielen, Marcel Ottens
2016-04-20 - Bioconjugate Chemistry
A Synthetic Carbohydrate Conjugate Vaccine Candidate against Shigellosis: Improved Bioconjugation and Impact of Alum on Immunogenicity
Robert M F van der Put, Tae Hee Kim, Catherine Guerreiro, Françoise Thouron, Peter Hoogerhout, Philippe J Sansonetti, Janny Westdijk, Michiel Stork, Armelle Phalipon, Laurence A Mulard
2015-11-27 - Vaccine
HPAEC-PAD quantification of Haemophilus influenzae type b polysaccharide in upstream and downstream samples
Robert M F van der Put, Alex de Haan, Jan G M van den IJssel, Ahd Hamidi, Michel Beurret
2014-11-13 - Human Vaccines & Immuno-therapeutics
Use of immuno assays during the development of a Hemophilus influenzae type b vaccine for technology transfer to emerging vaccine manufacturers
Ahd Hamidi, Hans Kreeftenberg
2014-11-01 - Human Vaccines & Immuno-therapeutics
Preclinical evaluation of a Haemophilus influenzae type b conjugate vaccine process intended for technology transfer
Ahd Hamidi, Pauline Verdijk, Hans Kreeftenberg
2014-07-16 - Vaccine
Lessons learned during the development and transfer of technology related to a new Hib conjugate vaccine to emerging vaccine manufacturers
A Hamidi, C Boog, S Jadhav, H Kreeftenberg
2013-05-17 - Biomedical Chromatography
HPAEC-PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide
Alex de Haan , Robert M. F. van der Put and Michel Beurret
Platforms OMV
2023-10-01 - ScienceDirect
Outer membrane vesicle-based intranasal vaccines
Peter Van der Ley, Virgil EJC Schijns,
2023-08-04 - Scientific Reports
In vitro alternative for reactogenicity assessment of outer membrane vesicle based vaccines In
Marijke W. A. Molenaar‑de Backer, Paulien Doodeman, Fereshte Rezai, Lisa M. Verhagen, Arno van der Ark, Els M. Plagmeijer, Bernard Metz, Naomi van Vlies, Olga Ophorst & René H. M. Raeven,
2022-12-20 - mSphere
Microencapsulated IL-12 Drives Genital Tract Immune Responses to Intranasal Gonococcal Outer Membrane Vesicle Vaccine and Induces Resistance to Vaginal Infection with Diverse Strains of Neisseria gonorrhoeae
Yingru Liu, Laura A Hammer, Jikke Daamen, Michiel Stork, Nejat K Egilmez, Michael W Russell
2021-12-01 - Analytical Chemistry
Validation of an FFF-MALS Method to Characterize the Production and Functionalization of Outer-Membrane Vesicles for Conjugate Vaccines
Robert M F van der Put , Arnoud Spies, Bernard Metz, Daniel Some, Roger Scherrers, Roland Pieters, Maarten Danial
2021-12-01 - Frontiers in Immunology
An Intranasal OMV-Based Vaccine Induces High Mucosal and Systemic Protecting Immunity Against a SARS-CoV-2 Infection
Peter van der Ley, Afshin Zariri, Elly van Riet, Dinja Oosterhoff, Corine Kruiswijk
2021-04-28 - Vaccine
Vaccination with meningococcal outer membrane vesicles carrying Borrelia OspA protects against experimental Lyme borreliosis
PM J Klouwens, M L M Salverda, J J Trentelman, J I Ersoz, A Wagemakers, M J H Gerritzen, P A van der Ley, J W Hovius
Describing the pre-clinical data of the candidate Lyme vaccine based on Intravacc’s OMV platform, this study shows that OMV’s expressing Borrelia (lipo)proteins is a promising vaccine concept that might lead to protecting humans against Lyme disease. Currently, no vaccine against Lyme disease, the fastest growing and most common tick-borne illness in North America and Europe, is available for humans. Antibiotics are the primary treatment and are usually successful, but only when given in time. A vaccine would limit the use of antibiotics and the potential rise of antibiotic resistance. For more information, see also our press release
2020-10-09 - Vaccine
Shortening the Lipid A Acyl Chains of Bordetella pertussis Enables Depletion of Lipopolysaccharide Endotoxic Activity
jesús Arenas, Elder Pupo, Coen Phielix, Dionne David, Afshin Zariri, Alla Zamyatina, Jan Tommassen, Peter van der Ley
2020-04-30 - Scientific Reports
Intranasal immunization with outer membrane vesicle pertussis vaccine confers broad protection through mucosal IgA and Th17 responses
René H M Raeven , Dedeke Rockx-Brouwer, Gaurav Kanojia, Larissa van der Maas, Tim H E Bindels, Rimko Ten Have, Elly van Riet, Bernard Metz
2019-11-01 - Applied Microbiology and Biotechnology
Continuous production of Neisseria meningitidis outer membrane vesicles
Matthias J H Gerritzen, Lilli Stangowez, Bas van de Waterbeemd, Dirk E Martens, René H Wijffels, Michiel Stork
2019-04-30 - International Journal of Pharmaceutics
Diphtheria toxoid dissolving microneedle vaccination: Adjuvant screening and effect of repeated-fractional dose administration
M Leone, S Romeijn, G Du, S E Le Dévédec, H Vrieling, C O’Mahony, J A Bouwstra, G Kersten
Vaccine development services
2022-12-20 - mSphere
Microencapsulated IL-12 Drives Genital Tract Immune Responses to Intranasal Gonococcal Outer Membrane Vesicle Vaccine and Induces Resistance to Vaginal Infection with Diverse Strains of Neisseria gonorrhoeae
Yingru Liu, Laura A Hammer, Jikke Daamen, Michiel Stork, Nejat K Egilmez, Michael W Russell
2022-09-30 - Vaccine X
Development of an animal component free production process for Sabin inactivated polio vaccine
Diego A Suarez-Zuluaga, Leo A van der Pol, Aart G van ‘t Oever, Wilfried A M Bakker, Yvonne E Thomassen
2022-07-05 - Vaccines
Mass Spectrometry-Based Quantification of the Antigens in Aluminum Hydroxide-Adjuvanted Diphtheria-Tetanus- Acellular-Pertussis Combination Vaccines
Larissa van der Maas, Maarten Danial, Gideon F. A. Kersten, Bernard Metz and Hugo D. Meiring
2021-07-05 - Emerging Microbes & Infections
Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells
Michiel M Kroes, Alberto Miranda-Bedate, Elise S Hovingh, Ronald Jacobi, Corrie Schot, Elder Pupo, René H M Raeven, Arno A J van der Ark, Jos P M van Putten, Jelle de Wit, Rob Mariman, Elena Pinelli
2021-06-16 - Scientific Reports
Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
Robin H G A van den Biggelaar , Larissa van der Maas, Hugo D Meiring, Jeroen L A Pennings, Willem van Eden, Victor P M G Rutten, Christine A Jansen
2021-06-02 - Journal of the American Society of Mass Spectrometry
Common Reference-Based Tandem Mass Tag Multiplexing for the Relative Quantification of Peptides: Design and Application to Degradome Analysis of Diphtheria Toxoid
Thomas J M Michiels, Madelief A van Veen, Hugo D Meiring, Wim Jiskoot, Gideon F A Kersten, Bernard Metz
2020-06-02 - Human Vaccines & Immuno-therapeutics
Prevalent levels of RSV serum neutralizing antibodies in healthy adults outside the RSV-season
Johan L Van Der Plas, Pauline Verdijk, Emilie M J Van Brummelen, Rienk E Jeeninga, Meta Roestenberg, Jacobus Burggraaf, Ingrid M C Kamerling
2020-05-01 - Journal of inherited metabolic disease
Slc22a5 haploinsufficiency does not aggravate the phenotype of the long-chain acyl-CoA dehydrogenase KO mouse
Pablo Ranea-Robles , Chunli Yu, Naomi van Vlies, Frédéric M Vaz, Sander M Houten
2020-01-17 - Human Vaccines & Immuno-therapeutics
In silico identification and modification of T cell epitopes in pertussis antigens associated with tolerance
Corine Kruiswijk, Guilhem Richard, Merijn L M Salverda, Pooja Hindocha, William D Martin, Anne S De Groot, Elly Van Riet
2022-10-12 Viruses
Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines
Wouter Johannes Petrus van den Braak, Bella Monica, Diana Limpens, Dedeke Rockx-Brouwer, Matthijn de Boer, Dinja Oosterhoff
Viruses
2022-10-12 - Viruses
Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines
Wouter Johannes Petrus van den Braak, Bella Monica, Diana Limpens, Dedeke Rockx-Brouwer, Matthijn de Boer, Dinja Oosterhoff